Pelosinus sp. UFO1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Negativicutes; Selenomonadales; Sporomusaceae; Pelosinus; unclassified Pelosinus

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4754 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A075KHE4|A0A075KHE4_9FIRM WbqC-like family protein OS=Pelosinus sp. UFO1 OX=484770 GN=UFO1_4096 PE=4 SV=1
MM1 pKa = 7.71ASTFVGLSIASSGLNAAQVGMTVTTNNMSNIDD33 pKa = 3.41TTGYY37 pKa = 9.9SRR39 pKa = 11.84QVVNQTSIGPAAVYY53 pKa = 10.45SSSLVGNGVEE63 pKa = 4.34VTSVDD68 pKa = 3.35SVRR71 pKa = 11.84SFRR74 pKa = 11.84LDD76 pKa = 3.12QKK78 pKa = 10.67YY79 pKa = 8.01WQEE82 pKa = 3.64NSAASLWEE90 pKa = 4.03AKK92 pKa = 8.75STYY95 pKa = 10.28LEE97 pKa = 3.92QAEE100 pKa = 4.72TILGSTDD107 pKa = 2.67TSYY110 pKa = 11.41ISTALDD116 pKa = 3.61TFNTALDD123 pKa = 3.98SLATDD128 pKa = 3.88PTSTSARR135 pKa = 11.84AVVLEE140 pKa = 4.24AAEE143 pKa = 4.85SVCSTLNDD151 pKa = 3.37ASSQLTQLRR160 pKa = 11.84SDD162 pKa = 3.42INSDD166 pKa = 3.07VKK168 pKa = 9.28TTVDD172 pKa = 3.8QINSYY177 pKa = 8.5ATQIAALNKK186 pKa = 10.07QITLATASGASTNEE200 pKa = 4.07LEE202 pKa = 4.42DD203 pKa = 3.64QRR205 pKa = 11.84GLLVDD210 pKa = 3.99EE211 pKa = 4.86LSGLVGCDD219 pKa = 2.96VTKK222 pKa = 10.96ADD224 pKa = 5.08DD225 pKa = 4.23GSLTISVEE233 pKa = 3.7GTTLVKK239 pKa = 10.77GNNAKK244 pKa = 10.04EE245 pKa = 4.13LEE247 pKa = 4.76CYY249 pKa = 9.06TVTDD253 pKa = 3.99TTSDD257 pKa = 3.15QYY259 pKa = 12.07GMYY262 pKa = 10.18GIRR265 pKa = 11.84WADD268 pKa = 3.21SGEE271 pKa = 4.39DD272 pKa = 3.7FDD274 pKa = 6.27SGDD277 pKa = 3.6SGALNGYY284 pKa = 10.06LEE286 pKa = 4.21VRR288 pKa = 11.84DD289 pKa = 4.45GNSSDD294 pKa = 3.68SKK296 pKa = 10.93GIPYY300 pKa = 10.25YY301 pKa = 10.77LSQLDD306 pKa = 3.91DD307 pKa = 3.69FARR310 pKa = 11.84TYY312 pKa = 11.59AEE314 pKa = 4.14AFNEE318 pKa = 4.52GVTSGTTTYY327 pKa = 10.43SGHH330 pKa = 6.8ADD332 pKa = 2.98GVGIDD337 pKa = 3.68EE338 pKa = 5.52AEE340 pKa = 4.06TTGIRR345 pKa = 11.84FFSYY349 pKa = 11.09DD350 pKa = 3.59DD351 pKa = 4.09LSSEE355 pKa = 4.33EE356 pKa = 4.35LMASGTDD363 pKa = 3.54TEE365 pKa = 5.29AVYY368 pKa = 11.02QNITAANISVSKK380 pKa = 10.52DD381 pKa = 3.0IQEE384 pKa = 4.4DD385 pKa = 4.03TNKK388 pKa = 10.07IATASSDD395 pKa = 3.61GEE397 pKa = 4.12EE398 pKa = 4.56SNTEE402 pKa = 3.91NLDD405 pKa = 4.29DD406 pKa = 5.49IISICSSVAISGNATVDD423 pKa = 3.65DD424 pKa = 4.93LYY426 pKa = 10.97STIIATVATDD436 pKa = 3.09SSYY439 pKa = 11.95AQVSYY444 pKa = 10.93NRR446 pKa = 11.84KK447 pKa = 9.41DD448 pKa = 3.75AIATYY453 pKa = 10.15IDD455 pKa = 3.57TSRR458 pKa = 11.84SSVSGVSSDD467 pKa = 3.68EE468 pKa = 3.92EE469 pKa = 4.73TVNLTIYY476 pKa = 9.86EE477 pKa = 4.22SAYY480 pKa = 10.02AASASVTSTWNEE492 pKa = 3.58IYY494 pKa = 7.88EE495 pKa = 4.51TTLNMVDD502 pKa = 4.1DD503 pKa = 4.43

Molecular weight: 53.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A075KK98|A0A075KK98_9FIRM Pyruvate water dikinase OS=Pelosinus sp. UFO1 OX=484770 GN=UFO1_4537 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.31QPNNLWRR12 pKa = 11.84KK13 pKa = 6.69RR14 pKa = 11.84THH16 pKa = 6.26GFRR19 pKa = 11.84EE20 pKa = 3.86RR21 pKa = 11.84MKK23 pKa = 10.49TRR25 pKa = 11.84GGQLVLKK32 pKa = 10.12RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.95IISAA44 pKa = 4.03

Molecular weight: 5.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4754

0

4754

1424518

30

3273

299.6

33.22

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.959 ± 0.036

1.155 ± 0.017

4.852 ± 0.029

6.38 ± 0.036

4.068 ± 0.027

7.373 ± 0.045

1.888 ± 0.017

8.283 ± 0.034

6.379 ± 0.033

9.685 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.018

4.473 ± 0.036

3.651 ± 0.023

3.643 ± 0.027

4.019 ± 0.028

5.977 ± 0.027

5.572 ± 0.029

7.448 ± 0.031

0.963 ± 0.014

3.317 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski