Candidatus Protochlamydia naegleriophila

Taxonomy: cellular organisms; Bacteria; PVC group; Chlamydiae; Chlamydiia; Parachlamydiales; Parachlamydiaceae; Candidatus Protochlamydia

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2571 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0U5EUD4|A0A0U5EUD4_9BACT Putative acylase and diesterase OS=Candidatus Protochlamydia naegleriophila OX=389348 GN=PNK_2258 PE=4 SV=1
MM1 pKa = 7.69TDD3 pKa = 3.61FPEE6 pKa = 4.18TLEE9 pKa = 4.97DD10 pKa = 3.81IVVGDD15 pKa = 3.64MNYY18 pKa = 10.35PSNEE22 pKa = 3.8TKK24 pKa = 10.53TFDD27 pKa = 3.27TTIYY31 pKa = 7.88MALYY35 pKa = 8.67PGHH38 pKa = 6.92FLGLIPSPTT47 pKa = 3.32

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0U5ERB9|A0A0U5ERB9_9BACT Uncharacterized protein OS=Candidatus Protochlamydia naegleriophila OX=389348 GN=PNK_1103 PE=4 SV=1
MM1 pKa = 7.0QADD4 pKa = 4.47AFQVIIDD11 pKa = 3.97YY12 pKa = 9.67PSLGVNCKK20 pKa = 10.27LSQSGLSNAFFQSSGQLKK38 pKa = 10.54SILKK42 pKa = 9.45GCSRR46 pKa = 11.84HH47 pKa = 5.14PFKK50 pKa = 11.06VRR52 pKa = 11.84YY53 pKa = 8.91YY54 pKa = 9.19SLKK57 pKa = 8.48SRR59 pKa = 11.84EE60 pKa = 3.86RR61 pKa = 11.84IGRR64 pKa = 11.84KK65 pKa = 8.21RR66 pKa = 11.84ALWLRR71 pKa = 11.84LCFCARR77 pKa = 11.84RR78 pKa = 11.84LFCPAARR85 pKa = 11.84CIGLSHH91 pKa = 6.53SVLSKK96 pKa = 10.08SCRR99 pKa = 3.42

Molecular weight: 11.22 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2571

0

2571

847202

29

2427

329.5

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.608 ± 0.045

1.276 ± 0.019

4.832 ± 0.033

6.664 ± 0.051

4.748 ± 0.034

6.09 ± 0.056

2.574 ± 0.026

6.789 ± 0.039

6.137 ± 0.041

11.148 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.208 ± 0.017

4.059 ± 0.035

4.345 ± 0.032

4.897 ± 0.038

4.648 ± 0.035

6.73 ± 0.041

4.969 ± 0.039

5.75 ± 0.044

1.231 ± 0.018

3.296 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski