Vibrio phage VSK

Taxonomy: Viruses; Monodnaviria; Loebvirae; Hofneiviricota; Faserviricetes; Tubulavirales; Inoviridae; Fibrovirus; Vibrio virus fs1

Average proteome isoelectric point is 7.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 14 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8SDJ2|Q8SDJ2_9VIRU Major coat protein OS=Vibrio phage VSK OX=181604 GN=orf49 PE=4 SV=1
MM1 pKa = 7.47SGFIAALNSDD11 pKa = 3.98NKK13 pKa = 10.36GANAPFFMVMFMRR26 pKa = 11.84KK27 pKa = 9.19LFIISLVISLYY38 pKa = 9.35FTALPEE44 pKa = 3.93FAAYY48 pKa = 9.47QLPFDD53 pKa = 3.86QTKK56 pKa = 8.61TFPTVQAAAEE66 pKa = 4.38YY67 pKa = 9.69YY68 pKa = 10.96ANLLGGTSCKK78 pKa = 10.4ADD80 pKa = 3.16GNRR83 pKa = 11.84FKK85 pKa = 10.61IYY87 pKa = 9.57RR88 pKa = 11.84VKK90 pKa = 10.74SISDD94 pKa = 3.33PSFVIFQQEE103 pKa = 4.26TYY105 pKa = 10.95LDD107 pKa = 3.7NKK109 pKa = 10.05CQVFSSKK116 pKa = 11.03GDD118 pKa = 3.25ISVTLIVVDD127 pKa = 5.79DD128 pKa = 3.96PTTPCEE134 pKa = 3.88ASKK137 pKa = 10.95GQTGKK142 pKa = 10.93VGWNSYY148 pKa = 7.76FWGSATPSRR157 pKa = 11.84DD158 pKa = 2.99ICSSALMGGCVALTGDD174 pKa = 4.41HH175 pKa = 6.66LCVNIDD181 pKa = 3.93PEE183 pKa = 4.44HH184 pKa = 6.85KK185 pKa = 9.6IDD187 pKa = 4.01PSLYY191 pKa = 8.87EE192 pKa = 4.39CEE194 pKa = 3.99ALYY197 pKa = 10.17IVQDD201 pKa = 4.32TPWSPSEE208 pKa = 3.88

Molecular weight: 22.89 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8W6D7|Q8W6D7_9VIRU Repressor 2 OS=Vibrio phage VSK OX=181604 GN=orf152 PE=4 SV=1
MM1 pKa = 7.54ANSARR6 pKa = 11.84NKK8 pKa = 9.97PLSQSVNPFVTIQLTSGALPRR29 pKa = 11.84FLAYY33 pKa = 10.25GGFNSDD39 pKa = 2.52GSQRR43 pKa = 11.84FSVTSVSTDD52 pKa = 2.81AHH54 pKa = 6.7KK55 pKa = 10.72MPLPTANAILPKK67 pKa = 10.13IKK69 pKa = 10.48RR70 pKa = 11.84NWPLAQISYY79 pKa = 8.36QVKK82 pKa = 9.77

Molecular weight: 8.93 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

14

0

14

2039

49

368

145.6

16.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.572 ± 0.433

1.864 ± 0.321

5.101 ± 0.482

4.61 ± 0.568

4.953 ± 0.277

5.787 ± 0.427

1.864 ± 0.455

7.062 ± 0.743

6.229 ± 0.479

9.269 ± 0.961

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.845 ± 0.324

4.365 ± 0.467

5.836 ± 0.514

3.678 ± 0.417

5.101 ± 0.813

8.043 ± 0.797

6.327 ± 0.695

6.13 ± 0.313

1.128 ± 0.164

3.237 ± 0.451
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski