Lewinella sp. W8

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Saprospiria; Saprospirales; Lewinellaceae; Lewinella; unclassified Lewinella

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4697 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7H5ETJ1|A0A7H5ETJ1_9BACT CHAT domain-containing protein OS=Lewinella sp. W8 OX=2528208 GN=E1J53_0004175 PE=4 SV=1
MM1 pKa = 7.62NYY3 pKa = 10.03LHH5 pKa = 7.03SLRR8 pKa = 11.84LLSGLFFVVLAFGGLSAQVTQDD30 pKa = 3.63FSTSTPGSHH39 pKa = 6.95HH40 pKa = 6.39YY41 pKa = 10.57VDD43 pKa = 4.25PLSPFTEE50 pKa = 5.36HH51 pKa = 7.31ILPATMAPQTQPNAATMGAIPGYY74 pKa = 9.57QVTYY78 pKa = 9.79TPTRR82 pKa = 11.84DD83 pKa = 3.11RR84 pKa = 11.84DD85 pKa = 3.69AGGGLTDD92 pKa = 4.0GEE94 pKa = 4.5LFGVVDD100 pKa = 3.82YY101 pKa = 10.0TVAPTPDD108 pKa = 3.41NDD110 pKa = 3.85MGSLTIDD117 pKa = 3.34IAANPPPSGNNNIYY131 pKa = 10.45VLQDD135 pKa = 3.38PDD137 pKa = 3.5GLATLRR143 pKa = 11.84FNPVVVDD150 pKa = 4.15GSTMFSMSYY159 pKa = 9.86IVANTSYY166 pKa = 11.14EE167 pKa = 4.17NGDD170 pKa = 3.63GADD173 pKa = 4.06DD174 pKa = 4.5RR175 pKa = 11.84IEE177 pKa = 4.24IYY179 pKa = 10.7LINTATGDD187 pKa = 3.3RR188 pKa = 11.84TMLFSAINEE197 pKa = 4.74AIPTTEE203 pKa = 3.25VWTTLTADD211 pKa = 3.93LSGLAGATVQLVIEE225 pKa = 4.83FDD227 pKa = 3.9TNASAEE233 pKa = 3.98EE234 pKa = 3.83MAIDD238 pKa = 4.56NIIFSTGVVEE248 pKa = 5.55ADD250 pKa = 2.81WPEE253 pKa = 4.04CTEE256 pKa = 4.22PSLAALGQSPATVCPGVPFRR276 pKa = 11.84MEE278 pKa = 3.46ISGSLGNATEE288 pKa = 4.05WVIYY292 pKa = 10.48SDD294 pKa = 4.65AEE296 pKa = 4.31GTQEE300 pKa = 4.14VARR303 pKa = 11.84TSSDD307 pKa = 3.07QYY309 pKa = 11.67DD310 pKa = 3.75FFGGAEE316 pKa = 4.41LGTTYY321 pKa = 10.68YY322 pKa = 10.84VRR324 pKa = 11.84GEE326 pKa = 4.28GGCASSMDD334 pKa = 4.0LTALALTIDD343 pKa = 5.39DD344 pKa = 5.1GDD346 pKa = 4.63CEE348 pKa = 4.48PNAPPSTSFEE358 pKa = 4.17DD359 pKa = 3.66ALASQEE365 pKa = 4.82DD366 pKa = 4.59YY367 pKa = 11.63VDD369 pKa = 3.26TGNPVIFRR377 pKa = 11.84FLEE380 pKa = 4.17NNQGQPTVTTGGRR393 pKa = 11.84GKK395 pKa = 10.39EE396 pKa = 3.91LGFITAFAPTRR407 pKa = 11.84LGTSGEE413 pKa = 4.15AGLTDD418 pKa = 4.05GDD420 pKa = 4.26ALGVTDD426 pKa = 5.86DD427 pKa = 4.46PDD429 pKa = 3.49AAFTDD434 pKa = 4.54GEE436 pKa = 4.38KK437 pKa = 11.18GFVFEE442 pKa = 4.88DD443 pKa = 3.51TDD445 pKa = 4.14GLVSAVFAPIDD456 pKa = 3.43IAGLSGVTVSFDD468 pKa = 3.13YY469 pKa = 10.58FIRR472 pKa = 11.84STSYY476 pKa = 10.08EE477 pKa = 4.09SSSTGTDD484 pKa = 2.36RR485 pKa = 11.84FTAVILNEE493 pKa = 4.24SFGLLEE499 pKa = 4.93EE500 pKa = 4.79IFDD503 pKa = 4.13AAADD507 pKa = 4.19GSGGFPDD514 pKa = 4.07LTIGQWTTVTYY525 pKa = 9.63TITEE529 pKa = 4.34SYY531 pKa = 10.71DD532 pKa = 3.16DD533 pKa = 5.24PIRR536 pKa = 11.84LTIQADD542 pKa = 3.62LDD544 pKa = 4.08AGSEE548 pKa = 4.19RR549 pKa = 11.84IFIDD553 pKa = 3.18NVSFSAGTVVCQDD566 pKa = 2.85EE567 pKa = 4.63AAPVIACPEE576 pKa = 4.87PISVDD581 pKa = 3.36VGADD585 pKa = 3.35CMATAEE591 pKa = 4.32FTATATDD598 pKa = 4.02DD599 pKa = 3.71CSSTVTITYY608 pKa = 10.12SQDD611 pKa = 2.79SGTNFPVGVTTVTATATDD629 pKa = 3.75EE630 pKa = 4.32TGKK633 pKa = 10.88SSTCTFDD640 pKa = 3.18VTVNDD645 pKa = 3.56VTAPTLDD652 pKa = 3.84CPEE655 pKa = 4.11VTVKK659 pKa = 10.73LDD661 pKa = 3.4STFSCDD667 pKa = 4.06FVVISNTDD675 pKa = 3.93FDD677 pKa = 6.04PIAADD682 pKa = 3.16NCEE685 pKa = 3.76GVTLLSANGLSSLVGDD701 pKa = 4.53TIFKK705 pKa = 7.96EE706 pKa = 4.57TTEE709 pKa = 4.29LTWTATDD716 pKa = 3.59ASGLTATCTQQINVIVPPSCFPTSVTEE743 pKa = 3.93QDD745 pKa = 3.36PSDD748 pKa = 3.78FANVVAIPNPFRR760 pKa = 11.84EE761 pKa = 3.94AVTINFEE768 pKa = 4.49LPFMDD773 pKa = 3.93EE774 pKa = 3.87VGIDD778 pKa = 3.57VLDD781 pKa = 3.45VSGRR785 pKa = 11.84VVQSARR791 pKa = 11.84VNPNGDD797 pKa = 2.95RR798 pKa = 11.84TYY800 pKa = 10.8SWRR803 pKa = 11.84WDD805 pKa = 3.38ATDD808 pKa = 3.35AAGGRR813 pKa = 11.84AAAGMYY819 pKa = 8.96FVRR822 pKa = 11.84LRR824 pKa = 11.84ARR826 pKa = 11.84GQLYY830 pKa = 7.84TKK832 pKa = 10.38RR833 pKa = 11.84IVLTRR838 pKa = 3.63

Molecular weight: 88.59 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7H5EYX6|A0A7H5EYX6_9BACT Glutamate--tRNA ligase OS=Lewinella sp. W8 OX=2528208 GN=gltX PE=3 SV=1
MM1 pKa = 7.59SKK3 pKa = 8.74TANKK7 pKa = 9.77IFLNVRR13 pKa = 11.84TSTWMRR19 pKa = 11.84ALTVVVMLGFGWQCYY34 pKa = 8.8HH35 pKa = 7.4LARR38 pKa = 11.84RR39 pKa = 11.84VDD41 pKa = 4.15FGEE44 pKa = 3.84LLARR48 pKa = 11.84LGSPSVWPWFVLVLAMMPLNWWLEE72 pKa = 3.84ARR74 pKa = 11.84KK75 pKa = 8.47WQLLLRR81 pKa = 11.84PFLAWPFAKK90 pKa = 9.72TLRR93 pKa = 11.84ATMVGVSLSAATPNRR108 pKa = 11.84IGEE111 pKa = 3.99IGGRR115 pKa = 11.84MLMGSRR121 pKa = 11.84EE122 pKa = 4.07EE123 pKa = 4.28AGSILTSSLLGSAAQWVAFLLLAWPALMWTAGDD156 pKa = 4.24LLADD160 pKa = 5.57RR161 pKa = 11.84IPFPVALLWPLGPGVLLLLWFGGKK185 pKa = 8.98PLLVKK190 pKa = 10.35IIRR193 pKa = 11.84WVSRR197 pKa = 11.84RR198 pKa = 11.84FSWNNAPLLEE208 pKa = 4.89GIRR211 pKa = 11.84EE212 pKa = 4.24VKK214 pKa = 10.22FGLMMRR220 pKa = 11.84AGAYY224 pKa = 9.48ACLRR228 pKa = 11.84FCVYY232 pKa = 10.48CMQLHH237 pKa = 6.47ILLGCFGLEE246 pKa = 4.03LPLLEE251 pKa = 4.51GMAGIAAIYY260 pKa = 9.19LVQAGIPLPPGLNLVTRR277 pKa = 11.84TEE279 pKa = 4.19LGLLLWNTEE288 pKa = 3.99PGAGIATVLAFGSLFAINVLLPALPAYY315 pKa = 8.73WLLVRR320 pKa = 11.84KK321 pKa = 10.19KK322 pKa = 10.26NLRR325 pKa = 3.62

Molecular weight: 36.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4697

0

4697

1781409

32

4222

379.3

42.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.49 ± 0.036

0.873 ± 0.024

6.122 ± 0.041

6.51 ± 0.037

4.599 ± 0.023

7.911 ± 0.043

1.871 ± 0.022

5.442 ± 0.026

3.803 ± 0.047

10.134 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.118 ± 0.019

4.183 ± 0.031

4.832 ± 0.025

3.691 ± 0.023

6.102 ± 0.047

5.774 ± 0.034

5.912 ± 0.05

6.722 ± 0.035

1.346 ± 0.014

3.564 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski