Amino acid dipepetide frequency for Bacillus phage Page

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.704AlaAla: 7.704 ± 0.882
0.629AlaCys: 0.629 ± 0.248
4.874AlaAsp: 4.874 ± 0.491
5.031AlaGlu: 5.031 ± 0.708
3.223AlaPhe: 3.223 ± 0.42
6.447AlaGly: 6.447 ± 0.79
0.786AlaHis: 0.786 ± 0.308
3.538AlaIle: 3.538 ± 0.573
4.796AlaLys: 4.796 ± 0.652
5.425AlaLeu: 5.425 ± 0.561
2.28AlaMet: 2.28 ± 0.369
3.145AlaAsn: 3.145 ± 0.607
3.145AlaPro: 3.145 ± 0.701
3.145AlaGln: 3.145 ± 0.732
4.245AlaArg: 4.245 ± 0.719
3.774AlaSer: 3.774 ± 0.743
4.717AlaThr: 4.717 ± 0.695
4.953AlaVal: 4.953 ± 0.649
0.786AlaTrp: 0.786 ± 0.264
3.223AlaTyr: 3.223 ± 0.449
0.0AlaXaa: 0.0 ± 0.0
Cys
0.393CysAla: 0.393 ± 0.178
0.314CysCys: 0.314 ± 0.193
0.314CysAsp: 0.314 ± 0.198
0.943CysGlu: 0.943 ± 0.307
0.157CysPhe: 0.157 ± 0.116
0.472CysGly: 0.472 ± 0.197
0.236CysHis: 0.236 ± 0.136
0.236CysIle: 0.236 ± 0.155
0.55CysLys: 0.55 ± 0.201
0.314CysLeu: 0.314 ± 0.142
0.314CysMet: 0.314 ± 0.173
0.472CysAsn: 0.472 ± 0.27
0.472CysPro: 0.472 ± 0.18
0.157CysGln: 0.157 ± 0.107
0.314CysArg: 0.314 ± 0.145
0.393CysSer: 0.393 ± 0.196
0.55CysThr: 0.55 ± 0.201
0.393CysVal: 0.393 ± 0.184
0.079CysTrp: 0.079 ± 0.073
0.157CysTyr: 0.157 ± 0.105
0.0CysXaa: 0.0 ± 0.0
Asp
3.695AspAla: 3.695 ± 0.511
0.629AspCys: 0.629 ± 0.298
3.538AspAsp: 3.538 ± 0.636
3.774AspGlu: 3.774 ± 0.46
3.066AspPhe: 3.066 ± 0.495
4.638AspGly: 4.638 ± 0.839
0.55AspHis: 0.55 ± 0.16
4.403AspIle: 4.403 ± 0.564
4.481AspLys: 4.481 ± 0.673
4.481AspLeu: 4.481 ± 0.533
1.572AspMet: 1.572 ± 0.454
2.987AspAsn: 2.987 ± 0.56
1.415AspPro: 1.415 ± 0.316
1.887AspGln: 1.887 ± 0.403
1.887AspArg: 1.887 ± 0.429
2.909AspSer: 2.909 ± 0.334
3.223AspThr: 3.223 ± 0.504
3.774AspVal: 3.774 ± 0.679
0.865AspTrp: 0.865 ± 0.335
2.516AspTyr: 2.516 ± 0.41
0.0AspXaa: 0.0 ± 0.0
Glu
5.031GluAla: 5.031 ± 0.803
0.629GluCys: 0.629 ± 0.19
2.83GluAsp: 2.83 ± 0.397
4.874GluGlu: 4.874 ± 0.823
2.201GluPhe: 2.201 ± 0.422
4.324GluGly: 4.324 ± 0.49
1.494GluHis: 1.494 ± 0.512
5.11GluIle: 5.11 ± 0.777
6.604GluLys: 6.604 ± 0.938
5.818GluLeu: 5.818 ± 0.967
2.752GluMet: 2.752 ± 0.567
4.167GluAsn: 4.167 ± 0.576
2.358GluPro: 2.358 ± 0.406
4.638GluGln: 4.638 ± 0.778
3.695GluArg: 3.695 ± 0.547
2.516GluSer: 2.516 ± 0.466
3.616GluThr: 3.616 ± 0.699
4.796GluVal: 4.796 ± 0.708
0.865GluTrp: 0.865 ± 0.262
2.516GluTyr: 2.516 ± 0.451
0.0GluXaa: 0.0 ± 0.0
Phe
1.887PheAla: 1.887 ± 0.338
0.314PheCys: 0.314 ± 0.166
2.358PheAsp: 2.358 ± 0.499
2.83PheGlu: 2.83 ± 0.428
1.887PhePhe: 1.887 ± 0.366
2.752PheGly: 2.752 ± 0.355
0.708PheHis: 0.708 ± 0.215
3.381PheIle: 3.381 ± 0.534
3.695PheLys: 3.695 ± 0.509
3.223PheLeu: 3.223 ± 0.424
1.336PheMet: 1.336 ± 0.295
3.538PheAsn: 3.538 ± 0.433
2.123PhePro: 2.123 ± 0.353
1.965PheGln: 1.965 ± 0.517
1.651PheArg: 1.651 ± 0.449
2.437PheSer: 2.437 ± 0.496
3.302PheThr: 3.302 ± 0.62
1.336PheVal: 1.336 ± 0.34
0.314PheTrp: 0.314 ± 0.141
0.865PheTyr: 0.865 ± 0.257
0.0PheXaa: 0.0 ± 0.0
Gly
6.053GlyAla: 6.053 ± 0.619
0.236GlyCys: 0.236 ± 0.127
2.752GlyAsp: 2.752 ± 0.467
4.245GlyGlu: 4.245 ± 0.635
2.673GlyPhe: 2.673 ± 0.497
5.739GlyGly: 5.739 ± 0.953
0.865GlyHis: 0.865 ± 0.235
3.459GlyIle: 3.459 ± 0.481
5.818GlyLys: 5.818 ± 0.987
5.66GlyLeu: 5.66 ± 0.737
2.673GlyMet: 2.673 ± 0.413
3.931GlyAsn: 3.931 ± 0.596
1.022GlyPro: 1.022 ± 0.288
2.909GlyGln: 2.909 ± 0.593
2.752GlyArg: 2.752 ± 0.467
4.088GlySer: 4.088 ± 0.768
4.953GlyThr: 4.953 ± 0.749
5.739GlyVal: 5.739 ± 0.736
0.943GlyTrp: 0.943 ± 0.309
2.594GlyTyr: 2.594 ± 0.44
0.0GlyXaa: 0.0 ± 0.0
His
1.022HisAla: 1.022 ± 0.349
0.314HisCys: 0.314 ± 0.178
1.336HisAsp: 1.336 ± 0.333
1.101HisGlu: 1.101 ± 0.408
0.943HisPhe: 0.943 ± 0.253
0.629HisGly: 0.629 ± 0.226
0.157HisHis: 0.157 ± 0.114
0.865HisIle: 0.865 ± 0.241
1.415HisLys: 1.415 ± 0.231
0.943HisLeu: 0.943 ± 0.238
0.314HisMet: 0.314 ± 0.172
1.022HisAsn: 1.022 ± 0.29
0.786HisPro: 0.786 ± 0.245
0.865HisGln: 0.865 ± 0.243
0.865HisArg: 0.865 ± 0.373
0.708HisSer: 0.708 ± 0.207
0.708HisThr: 0.708 ± 0.21
0.786HisVal: 0.786 ± 0.28
0.472HisTrp: 0.472 ± 0.217
1.022HisTyr: 1.022 ± 0.264
0.0HisXaa: 0.0 ± 0.0
Ile
4.403IleAla: 4.403 ± 0.673
0.314IleCys: 0.314 ± 0.167
4.796IleAsp: 4.796 ± 0.86
4.245IleGlu: 4.245 ± 0.701
1.965IlePhe: 1.965 ± 0.496
3.145IleGly: 3.145 ± 0.486
1.494IleHis: 1.494 ± 0.381
3.774IleIle: 3.774 ± 0.734
5.975IleLys: 5.975 ± 0.764
3.302IleLeu: 3.302 ± 0.59
1.415IleMet: 1.415 ± 0.339
3.852IleAsn: 3.852 ± 0.581
2.123IlePro: 2.123 ± 0.331
2.673IleGln: 2.673 ± 0.425
2.123IleArg: 2.123 ± 0.455
2.673IleSer: 2.673 ± 0.559
3.852IleThr: 3.852 ± 0.684
3.774IleVal: 3.774 ± 0.474
0.472IleTrp: 0.472 ± 0.186
2.28IleTyr: 2.28 ± 0.45
0.0IleXaa: 0.0 ± 0.0
Lys
6.368LysAla: 6.368 ± 0.931
0.786LysCys: 0.786 ± 0.273
4.167LysAsp: 4.167 ± 0.531
6.918LysGlu: 6.918 ± 0.864
2.909LysPhe: 2.909 ± 0.395
5.425LysGly: 5.425 ± 0.739
1.022LysHis: 1.022 ± 0.336
4.088LysIle: 4.088 ± 0.714
7.547LysLys: 7.547 ± 0.897
5.346LysLeu: 5.346 ± 0.695
2.909LysMet: 2.909 ± 0.467
5.896LysAsn: 5.896 ± 0.619
2.437LysPro: 2.437 ± 0.412
4.796LysGln: 4.796 ± 0.802
4.245LysArg: 4.245 ± 0.469
4.874LysSer: 4.874 ± 0.81
4.403LysThr: 4.403 ± 0.598
4.953LysVal: 4.953 ± 0.643
0.472LysTrp: 0.472 ± 0.191
3.302LysTyr: 3.302 ± 0.567
0.0LysXaa: 0.0 ± 0.0
Leu
4.56LeuAla: 4.56 ± 0.649
0.157LeuCys: 0.157 ± 0.131
3.695LeuAsp: 3.695 ± 0.496
6.132LeuGlu: 6.132 ± 0.836
2.516LeuPhe: 2.516 ± 0.457
3.931LeuGly: 3.931 ± 0.62
1.022LeuHis: 1.022 ± 0.309
3.616LeuIle: 3.616 ± 0.779
5.896LeuLys: 5.896 ± 0.702
5.031LeuLeu: 5.031 ± 0.796
2.358LeuMet: 2.358 ± 0.399
6.211LeuAsn: 6.211 ± 0.693
1.808LeuPro: 1.808 ± 0.447
2.673LeuGln: 2.673 ± 0.47
2.987LeuArg: 2.987 ± 0.524
5.896LeuSer: 5.896 ± 0.589
5.582LeuThr: 5.582 ± 0.937
4.088LeuVal: 4.088 ± 0.563
1.101LeuTrp: 1.101 ± 0.305
2.594LeuTyr: 2.594 ± 0.641
0.0LeuXaa: 0.0 ± 0.0
Met
1.887MetAla: 1.887 ± 0.418
0.236MetCys: 0.236 ± 0.137
1.887MetAsp: 1.887 ± 0.348
1.101MetGlu: 1.101 ± 0.312
1.415MetPhe: 1.415 ± 0.354
1.572MetGly: 1.572 ± 0.516
0.236MetHis: 0.236 ± 0.14
1.179MetIle: 1.179 ± 0.348
2.516MetLys: 2.516 ± 0.472
2.044MetLeu: 2.044 ± 0.55
0.629MetMet: 0.629 ± 0.224
2.123MetAsn: 2.123 ± 0.385
1.887MetPro: 1.887 ± 0.4
1.651MetGln: 1.651 ± 0.347
1.022MetArg: 1.022 ± 0.346
1.887MetSer: 1.887 ± 0.322
2.044MetThr: 2.044 ± 0.597
2.123MetVal: 2.123 ± 0.333
0.079MetTrp: 0.079 ± 0.079
1.258MetTyr: 1.258 ± 0.477
0.0MetXaa: 0.0 ± 0.0
Asn
5.975AsnAla: 5.975 ± 0.792
0.393AsnCys: 0.393 ± 0.194
3.616AsnAsp: 3.616 ± 0.737
3.931AsnGlu: 3.931 ± 0.661
2.83AsnPhe: 2.83 ± 0.443
4.245AsnGly: 4.245 ± 0.566
1.494AsnHis: 1.494 ± 0.462
3.459AsnIle: 3.459 ± 0.526
4.324AsnLys: 4.324 ± 0.61
3.302AsnLeu: 3.302 ± 0.558
1.651AsnMet: 1.651 ± 0.415
3.774AsnAsn: 3.774 ± 1.104
2.83AsnPro: 2.83 ± 0.872
2.83AsnGln: 2.83 ± 0.489
3.145AsnArg: 3.145 ± 0.498
3.066AsnSer: 3.066 ± 0.41
3.302AsnThr: 3.302 ± 0.501
4.717AsnVal: 4.717 ± 0.926
0.708AsnTrp: 0.708 ± 0.268
2.516AsnTyr: 2.516 ± 0.565
0.0AsnXaa: 0.0 ± 0.0
Pro
3.145ProAla: 3.145 ± 0.487
0.236ProCys: 0.236 ± 0.12
1.965ProAsp: 1.965 ± 0.485
2.909ProGlu: 2.909 ± 0.565
1.808ProPhe: 1.808 ± 0.351
2.516ProGly: 2.516 ± 0.444
0.314ProHis: 0.314 ± 0.156
2.044ProIle: 2.044 ± 0.53
2.594ProLys: 2.594 ± 0.477
2.594ProLeu: 2.594 ± 0.469
0.629ProMet: 0.629 ± 0.207
1.572ProAsn: 1.572 ± 0.362
1.336ProPro: 1.336 ± 0.692
1.965ProGln: 1.965 ± 0.389
1.336ProArg: 1.336 ± 0.28
2.044ProSer: 2.044 ± 0.298
2.673ProThr: 2.673 ± 0.697
2.28ProVal: 2.28 ± 0.435
0.393ProTrp: 0.393 ± 0.166
1.73ProTyr: 1.73 ± 0.52
0.0ProXaa: 0.0 ± 0.0
Gln
4.167GlnAla: 4.167 ± 0.603
0.079GlnCys: 0.079 ± 0.07
2.909GlnAsp: 2.909 ± 0.468
3.616GlnGlu: 3.616 ± 0.621
2.437GlnPhe: 2.437 ± 0.693
3.852GlnGly: 3.852 ± 0.626
0.55GlnHis: 0.55 ± 0.244
1.965GlnIle: 1.965 ± 0.371
3.145GlnLys: 3.145 ± 0.698
4.56GlnLeu: 4.56 ± 0.576
1.101GlnMet: 1.101 ± 0.361
3.774GlnAsn: 3.774 ± 0.749
1.336GlnPro: 1.336 ± 0.428
4.796GlnGln: 4.796 ± 1.473
2.437GlnArg: 2.437 ± 0.712
2.516GlnSer: 2.516 ± 0.481
2.201GlnThr: 2.201 ± 0.346
1.965GlnVal: 1.965 ± 0.503
1.336GlnTrp: 1.336 ± 0.562
1.651GlnTyr: 1.651 ± 0.47
0.0GlnXaa: 0.0 ± 0.0
Arg
3.538ArgAla: 3.538 ± 0.67
0.55ArgCys: 0.55 ± 0.213
2.28ArgAsp: 2.28 ± 0.511
4.009ArgGlu: 4.009 ± 0.679
2.123ArgPhe: 2.123 ± 0.401
2.909ArgGly: 2.909 ± 0.583
0.865ArgHis: 0.865 ± 0.357
2.594ArgIle: 2.594 ± 0.368
3.066ArgLys: 3.066 ± 0.404
3.616ArgLeu: 3.616 ± 0.563
1.101ArgMet: 1.101 ± 0.238
2.28ArgAsn: 2.28 ± 0.416
0.472ArgPro: 0.472 ± 0.203
2.201ArgGln: 2.201 ± 0.495
1.73ArgArg: 1.73 ± 0.388
2.123ArgSer: 2.123 ± 0.39
1.73ArgThr: 1.73 ± 0.338
3.066ArgVal: 3.066 ± 0.405
0.314ArgTrp: 0.314 ± 0.166
1.651ArgTyr: 1.651 ± 0.365
0.0ArgXaa: 0.0 ± 0.0
Ser
3.931SerAla: 3.931 ± 0.508
0.314SerCys: 0.314 ± 0.146
2.987SerAsp: 2.987 ± 0.497
3.381SerGlu: 3.381 ± 0.532
2.673SerPhe: 2.673 ± 0.429
5.189SerGly: 5.189 ± 0.675
1.022SerHis: 1.022 ± 0.327
3.852SerIle: 3.852 ± 0.479
5.11SerLys: 5.11 ± 0.545
4.167SerLeu: 4.167 ± 0.525
1.572SerMet: 1.572 ± 0.399
2.752SerAsn: 2.752 ± 0.529
2.201SerPro: 2.201 ± 0.504
3.066SerGln: 3.066 ± 0.773
1.494SerArg: 1.494 ± 0.348
4.717SerSer: 4.717 ± 0.823
2.83SerThr: 2.83 ± 0.627
2.987SerVal: 2.987 ± 0.537
0.708SerTrp: 0.708 ± 0.174
2.123SerTyr: 2.123 ± 0.384
0.0SerXaa: 0.0 ± 0.0
Thr
4.717ThrAla: 4.717 ± 0.666
0.157ThrCys: 0.157 ± 0.125
2.752ThrAsp: 2.752 ± 0.518
3.931ThrGlu: 3.931 ± 0.573
2.437ThrPhe: 2.437 ± 0.407
4.167ThrGly: 4.167 ± 0.509
1.101ThrHis: 1.101 ± 0.334
4.717ThrIle: 4.717 ± 0.914
5.031ThrLys: 5.031 ± 0.546
3.852ThrLeu: 3.852 ± 0.454
1.022ThrMet: 1.022 ± 0.305
3.538ThrAsn: 3.538 ± 0.584
3.852ThrPro: 3.852 ± 0.629
2.987ThrGln: 2.987 ± 0.436
1.572ThrArg: 1.572 ± 0.255
3.459ThrSer: 3.459 ± 0.642
3.695ThrThr: 3.695 ± 0.693
4.088ThrVal: 4.088 ± 0.856
0.629ThrTrp: 0.629 ± 0.242
2.673ThrTyr: 2.673 ± 0.522
0.0ThrXaa: 0.0 ± 0.0
Val
4.56ValAla: 4.56 ± 0.642
0.393ValCys: 0.393 ± 0.25
4.403ValAsp: 4.403 ± 0.539
4.245ValGlu: 4.245 ± 0.72
2.673ValPhe: 2.673 ± 0.552
4.088ValGly: 4.088 ± 0.7
1.415ValHis: 1.415 ± 0.269
3.302ValIle: 3.302 ± 0.554
5.267ValLys: 5.267 ± 0.687
4.56ValLeu: 4.56 ± 0.695
1.965ValMet: 1.965 ± 0.349
3.459ValAsn: 3.459 ± 0.625
2.673ValPro: 2.673 ± 0.498
2.28ValGln: 2.28 ± 0.402
2.201ValArg: 2.201 ± 0.354
4.481ValSer: 4.481 ± 0.604
4.009ValThr: 4.009 ± 0.523
2.752ValVal: 2.752 ± 0.634
0.393ValTrp: 0.393 ± 0.187
2.358ValTyr: 2.358 ± 0.45
0.0ValXaa: 0.0 ± 0.0
Trp
0.629TrpAla: 0.629 ± 0.197
0.079TrpCys: 0.079 ± 0.072
0.472TrpAsp: 0.472 ± 0.183
0.865TrpGlu: 0.865 ± 0.319
0.393TrpPhe: 0.393 ± 0.198
0.55TrpGly: 0.55 ± 0.229
0.157TrpHis: 0.157 ± 0.117
0.472TrpIle: 0.472 ± 0.223
1.494TrpLys: 1.494 ± 0.43
1.101TrpLeu: 1.101 ± 0.292
0.236TrpMet: 0.236 ± 0.127
1.101TrpAsn: 1.101 ± 0.313
0.236TrpPro: 0.236 ± 0.162
1.179TrpGln: 1.179 ± 0.59
0.55TrpArg: 0.55 ± 0.23
0.708TrpSer: 0.708 ± 0.319
0.55TrpThr: 0.55 ± 0.216
0.55TrpVal: 0.55 ± 0.193
0.079TrpTrp: 0.079 ± 0.07
0.472TrpTyr: 0.472 ± 0.19
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.201TyrAla: 2.201 ± 0.327
0.472TyrCys: 0.472 ± 0.248
2.358TyrAsp: 2.358 ± 0.416
2.752TyrGlu: 2.752 ± 0.552
1.572TyrPhe: 1.572 ± 0.446
2.516TyrGly: 2.516 ± 0.508
0.786TyrHis: 0.786 ± 0.242
2.594TyrIle: 2.594 ± 0.612
3.538TyrLys: 3.538 ± 0.653
2.594TyrLeu: 2.594 ± 0.395
0.865TyrMet: 0.865 ± 0.283
2.516TyrAsn: 2.516 ± 0.468
1.572TyrPro: 1.572 ± 0.344
1.73TyrGln: 1.73 ± 0.353
1.965TyrArg: 1.965 ± 0.343
1.808TyrSer: 1.808 ± 0.428
2.437TyrThr: 2.437 ± 0.425
2.358TyrVal: 2.358 ± 0.489
0.865TyrTrp: 0.865 ± 0.224
1.258TyrTyr: 1.258 ± 0.321
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 50 proteins (12721 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski