Muribaculaceae bacterium Isolate-002 (NCI)

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Muribaculaceae; unclassified Muribaculaceae

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2861 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q0J4L7|A0A4Q0J4L7_9BACT DUF3298 domain-containing protein OS=Muribaculaceae bacterium Isolate-002 (NCI) OX=2489215 GN=ED352_04770 PE=4 SV=1
MM1 pKa = 7.23YY2 pKa = 10.52NDD4 pKa = 3.01IVRR7 pKa = 11.84FFTDD11 pKa = 5.07LIEE14 pKa = 4.12QSGSYY19 pKa = 10.4DD20 pKa = 2.89IARR23 pKa = 11.84AEE25 pKa = 4.26FHH27 pKa = 7.3RR28 pKa = 11.84IMADD32 pKa = 3.31DD33 pKa = 5.63DD34 pKa = 4.66DD35 pKa = 5.34LHH37 pKa = 6.35EE38 pKa = 5.66QYY40 pKa = 11.04CEE42 pKa = 3.78WCDD45 pKa = 3.37EE46 pKa = 4.22NGYY49 pKa = 8.36SEE51 pKa = 5.81RR52 pKa = 11.84NGFDD56 pKa = 2.98EE57 pKa = 7.05FIDD60 pKa = 4.04QRR62 pKa = 11.84IDD64 pKa = 3.2SHH66 pKa = 8.44DD67 pKa = 4.23SVWNTLNDD75 pKa = 3.41YY76 pKa = 11.42DD77 pKa = 4.25EE78 pKa = 4.47

Molecular weight: 9.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q0J029|A0A4Q0J029_9BACT DUF4134 domain-containing protein OS=Muribaculaceae bacterium Isolate-002 (NCI) OX=2489215 GN=ED352_07125 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 10.23VITMEE7 pKa = 4.02SSAFRR12 pKa = 11.84SLTEE16 pKa = 3.93QIAEE20 pKa = 4.05IAAHH24 pKa = 5.66VRR26 pKa = 11.84AASGDD31 pKa = 3.61KK32 pKa = 10.39KK33 pKa = 10.73AASPDD38 pKa = 3.27RR39 pKa = 11.84LLTTRR44 pKa = 11.84EE45 pKa = 3.83AAHH48 pKa = 6.7LLNVSTRR55 pKa = 11.84TLQRR59 pKa = 11.84MRR61 pKa = 11.84SEE63 pKa = 3.67QRR65 pKa = 11.84IGYY68 pKa = 8.75VVLRR72 pKa = 11.84GKK74 pKa = 10.05CRR76 pKa = 11.84YY77 pKa = 8.39RR78 pKa = 11.84QSEE81 pKa = 3.86IGRR84 pKa = 11.84LLEE87 pKa = 4.15ACAVNEE93 pKa = 4.46DD94 pKa = 3.58AATPQEE100 pKa = 4.3LKK102 pKa = 10.6RR103 pKa = 11.84NHH105 pKa = 5.62TLRR108 pKa = 11.84TGGKK112 pKa = 8.07PKK114 pKa = 10.3GRR116 pKa = 11.84RR117 pKa = 11.84TT118 pKa = 3.39

Molecular weight: 13.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2861

0

2861

936511

23

6033

327.3

36.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.488 ± 0.052

1.229 ± 0.021

6.312 ± 0.038

6.037 ± 0.05

4.154 ± 0.03

7.1 ± 0.047

1.863 ± 0.019

6.769 ± 0.046

5.356 ± 0.042

8.582 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.711 ± 0.021

4.753 ± 0.035

3.999 ± 0.027

2.96 ± 0.031

5.533 ± 0.04

6.408 ± 0.041

5.802 ± 0.031

6.752 ± 0.037

1.182 ± 0.02

4.009 ± 0.036
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski