Kingella negevensis

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Neisseriales; Neisseriaceae; Kingella

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A238HI21|A0A238HI21_9NEIS Isoform of A0A238HG87 ATP synthase subunit c OS=Kingella negevensis OX=1522312 GN=atpE PE=3 SV=1
MM1 pKa = 7.7CFLDD5 pKa = 4.78NGFCTRR11 pKa = 11.84EE12 pKa = 3.63RR13 pKa = 11.84FFDD16 pKa = 3.44EE17 pKa = 4.94RR18 pKa = 11.84EE19 pKa = 4.19CVDD22 pKa = 3.65PNDD25 pKa = 4.26FVAQLGGEE33 pKa = 4.51LSPANAASLLDD44 pKa = 3.6MMNGAEE50 pKa = 4.13ITEE53 pKa = 4.76LLDD56 pKa = 5.03DD57 pKa = 4.77MNAPDD62 pKa = 5.84CGWQYY67 pKa = 11.5LGNFCRR73 pKa = 11.84AII75 pKa = 3.65

Molecular weight:
8.39 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A238TG83|A0A238TG83_9NEIS Isoform of A0A238HFG4 Uncharacterized protein OS=Kingella negevensis OX=1522312 GN=KEBURONENSIS_00691 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.91RR14 pKa = 11.84THH16 pKa = 5.89GFLVRR21 pKa = 11.84SKK23 pKa = 9.38TRR25 pKa = 11.84GGRR28 pKa = 11.84AVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.87GRR39 pKa = 11.84KK40 pKa = 8.75RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight:
5.05 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

359

1741

2100

596964

30

2511

284.3

31.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.881 ± 0.068

1.027 ± 0.02

5.027 ± 0.045

6.08 ± 0.062

4.195 ± 0.04

6.747 ± 0.063

2.291 ± 0.031

5.952 ± 0.053

5.669 ± 0.044

9.94 ± 0.064

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.587 ± 0.03

4.684 ± 0.05

4.02 ± 0.037

4.95 ± 0.048

4.736 ± 0.049

5.682 ± 0.043

5.515 ± 0.045

6.769 ± 0.049

1.271 ± 0.023

2.977 ± 0.036

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski