Amino acid dipepetide frequency for Shigella phage phi25-307

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.945AlaAla: 4.945 ± 0.392
0.361AlaCys: 0.361 ± 0.081
4.07AlaAsp: 4.07 ± 0.305
5.268AlaGlu: 5.268 ± 0.388
2.263AlaPhe: 2.263 ± 0.196
4.375AlaGly: 4.375 ± 0.378
1.198AlaHis: 1.198 ± 0.177
4.983AlaIle: 4.983 ± 0.294
5.326AlaLys: 5.326 ± 0.307
6.048AlaLeu: 6.048 ± 0.349
1.445AlaMet: 1.445 ± 0.168
3.424AlaAsn: 3.424 ± 0.27
2.454AlaPro: 2.454 ± 0.198
2.454AlaGln: 2.454 ± 0.262
3.024AlaArg: 3.024 ± 0.263
5.059AlaSer: 5.059 ± 0.339
3.595AlaThr: 3.595 ± 0.399
4.413AlaVal: 4.413 ± 0.277
0.97AlaTrp: 0.97 ± 0.129
2.625AlaTyr: 2.625 ± 0.225
0.0AlaXaa: 0.0 ± 0.0
Cys
0.666CysAla: 0.666 ± 0.12
0.209CysCys: 0.209 ± 0.073
0.59CysAsp: 0.59 ± 0.112
0.761CysGlu: 0.761 ± 0.137
0.437CysPhe: 0.437 ± 0.106
0.799CysGly: 0.799 ± 0.126
0.285CysHis: 0.285 ± 0.067
0.647CysIle: 0.647 ± 0.106
0.666CysLys: 0.666 ± 0.122
0.59CysLeu: 0.59 ± 0.113
0.209CysMet: 0.209 ± 0.081
0.571CysAsn: 0.571 ± 0.11
0.609CysPro: 0.609 ± 0.11
0.418CysGln: 0.418 ± 0.086
0.685CysArg: 0.685 ± 0.105
0.78CysSer: 0.78 ± 0.152
0.514CysThr: 0.514 ± 0.104
0.571CysVal: 0.571 ± 0.102
0.152CysTrp: 0.152 ± 0.06
0.475CysTyr: 0.475 ± 0.088
0.0CysXaa: 0.0 ± 0.0
Asp
4.755AspAla: 4.755 ± 0.321
0.609AspCys: 0.609 ± 0.094
3.975AspAsp: 3.975 ± 0.304
4.622AspGlu: 4.622 ± 0.381
3.252AspPhe: 3.252 ± 0.241
5.078AspGly: 5.078 ± 0.368
0.818AspHis: 0.818 ± 0.126
4.869AspIle: 4.869 ± 0.31
4.108AspLys: 4.108 ± 0.311
4.945AspLeu: 4.945 ± 0.36
1.655AspMet: 1.655 ± 0.178
2.777AspAsn: 2.777 ± 0.202
2.092AspPro: 2.092 ± 0.231
1.445AspGln: 1.445 ± 0.148
2.187AspArg: 2.187 ± 0.215
3.785AspSer: 3.785 ± 0.29
3.309AspThr: 3.309 ± 0.317
4.565AspVal: 4.565 ± 0.341
1.141AspTrp: 1.141 ± 0.169
3.271AspTyr: 3.271 ± 0.262
0.0AspXaa: 0.0 ± 0.0
Glu
5.345GluAla: 5.345 ± 0.418
1.084GluCys: 1.084 ± 0.159
4.451GluAsp: 4.451 ± 0.32
4.888GluGlu: 4.888 ± 0.35
3.785GluPhe: 3.785 ± 0.289
3.652GluGly: 3.652 ± 0.305
1.141GluHis: 1.141 ± 0.176
6.257GluIle: 6.257 ± 0.377
4.66GluLys: 4.66 ± 0.357
6.181GluLeu: 6.181 ± 0.399
2.149GluMet: 2.149 ± 0.195
3.747GluAsn: 3.747 ± 0.295
1.75GluPro: 1.75 ± 0.196
2.511GluGln: 2.511 ± 0.243
2.891GluArg: 2.891 ± 0.246
3.918GluSer: 3.918 ± 0.267
4.222GluThr: 4.222 ± 0.316
4.85GluVal: 4.85 ± 0.25
0.97GluTrp: 0.97 ± 0.127
3.424GluTyr: 3.424 ± 0.286
0.0GluXaa: 0.0 ± 0.0
Phe
2.891PheAla: 2.891 ± 0.259
0.437PheCys: 0.437 ± 0.086
3.195PheAsp: 3.195 ± 0.238
3.576PheGlu: 3.576 ± 0.262
1.369PhePhe: 1.369 ± 0.155
2.625PheGly: 2.625 ± 0.189
0.628PheHis: 0.628 ± 0.109
3.081PheIle: 3.081 ± 0.274
4.127PheLys: 4.127 ± 0.331
2.416PheLeu: 2.416 ± 0.203
1.141PheMet: 1.141 ± 0.158
2.948PheAsn: 2.948 ± 0.239
1.027PhePro: 1.027 ± 0.13
1.274PheGln: 1.274 ± 0.168
1.997PheArg: 1.997 ± 0.196
3.1PheSer: 3.1 ± 0.224
2.511PheThr: 2.511 ± 0.216
3.043PheVal: 3.043 ± 0.238
0.533PheTrp: 0.533 ± 0.094
1.94PheTyr: 1.94 ± 0.191
0.0PheXaa: 0.0 ± 0.0
Gly
3.157GlyAla: 3.157 ± 0.28
0.533GlyCys: 0.533 ± 0.108
3.956GlyAsp: 3.956 ± 0.283
3.709GlyGlu: 3.709 ± 0.218
2.587GlyPhe: 2.587 ± 0.229
3.557GlyGly: 3.557 ± 0.272
0.97GlyHis: 0.97 ± 0.151
4.47GlyIle: 4.47 ± 0.312
4.432GlyLys: 4.432 ± 0.319
5.402GlyLeu: 5.402 ± 0.358
1.636GlyMet: 1.636 ± 0.211
3.271GlyAsn: 3.271 ± 0.383
1.845GlyPro: 1.845 ± 0.17
2.149GlyGln: 2.149 ± 0.224
2.796GlyArg: 2.796 ± 0.223
4.26GlySer: 4.26 ± 0.353
4.508GlyThr: 4.508 ± 0.437
3.918GlyVal: 3.918 ± 0.284
0.818GlyTrp: 0.818 ± 0.111
3.214GlyTyr: 3.214 ± 0.243
0.0GlyXaa: 0.0 ± 0.0
His
0.913HisAla: 0.913 ± 0.114
0.304HisCys: 0.304 ± 0.078
1.046HisAsp: 1.046 ± 0.149
1.103HisGlu: 1.103 ± 0.159
0.856HisPhe: 0.856 ± 0.134
0.951HisGly: 0.951 ± 0.156
0.323HisHis: 0.323 ± 0.072
1.407HisIle: 1.407 ± 0.143
1.16HisLys: 1.16 ± 0.179
1.198HisLeu: 1.198 ± 0.158
0.399HisMet: 0.399 ± 0.093
0.799HisAsn: 0.799 ± 0.119
0.875HisPro: 0.875 ± 0.141
0.552HisGln: 0.552 ± 0.088
0.837HisArg: 0.837 ± 0.141
1.16HisSer: 1.16 ± 0.199
1.027HisThr: 1.027 ± 0.203
1.046HisVal: 1.046 ± 0.148
0.171HisTrp: 0.171 ± 0.064
0.609HisTyr: 0.609 ± 0.111
0.0HisXaa: 0.0 ± 0.0
Ile
4.983IleAla: 4.983 ± 0.333
0.723IleCys: 0.723 ± 0.112
5.078IleAsp: 5.078 ± 0.316
5.211IleGlu: 5.211 ± 0.335
2.53IlePhe: 2.53 ± 0.207
4.241IleGly: 4.241 ± 0.28
1.084IleHis: 1.084 ± 0.145
4.812IleIle: 4.812 ± 0.364
6.942IleLys: 6.942 ± 0.407
4.241IleLeu: 4.241 ± 0.282
1.636IleMet: 1.636 ± 0.178
4.888IleAsn: 4.888 ± 0.31
2.644IlePro: 2.644 ± 0.229
2.568IleGln: 2.568 ± 0.228
3.328IleArg: 3.328 ± 0.287
4.565IleSer: 4.565 ± 0.27
5.078IleThr: 5.078 ± 0.319
4.413IleVal: 4.413 ± 0.312
0.628IleTrp: 0.628 ± 0.111
2.625IleTyr: 2.625 ± 0.261
0.0IleXaa: 0.0 ± 0.0
Lys
6.124LysAla: 6.124 ± 0.449
0.704LysCys: 0.704 ± 0.116
4.451LysAsp: 4.451 ± 0.307
5.915LysGlu: 5.915 ± 0.434
3.652LysPhe: 3.652 ± 0.257
4.546LysGly: 4.546 ± 0.259
1.503LysHis: 1.503 ± 0.175
5.059LysIle: 5.059 ± 0.355
4.736LysLys: 4.736 ± 0.375
6.41LysLeu: 6.41 ± 0.383
2.301LysMet: 2.301 ± 0.209
4.222LysAsn: 4.222 ± 0.312
2.473LysPro: 2.473 ± 0.218
2.377LysGln: 2.377 ± 0.207
3.233LysArg: 3.233 ± 0.242
4.203LysSer: 4.203 ± 0.267
4.508LysThr: 4.508 ± 0.303
4.945LysVal: 4.945 ± 0.325
1.293LysTrp: 1.293 ± 0.127
3.386LysTyr: 3.386 ± 0.26
0.0LysXaa: 0.0 ± 0.0
Leu
5.287LeuAla: 5.287 ± 0.334
0.875LeuCys: 0.875 ± 0.136
5.268LeuAsp: 5.268 ± 0.324
5.116LeuGlu: 5.116 ± 0.435
3.29LeuPhe: 3.29 ± 0.297
4.013LeuGly: 4.013 ± 0.279
1.103LeuHis: 1.103 ± 0.152
4.945LeuIle: 4.945 ± 0.322
5.763LeuLys: 5.763 ± 0.375
5.021LeuLeu: 5.021 ± 0.29
2.13LeuMet: 2.13 ± 0.223
4.869LeuAsn: 4.869 ± 0.307
2.986LeuPro: 2.986 ± 0.217
2.72LeuGln: 2.72 ± 0.221
3.633LeuArg: 3.633 ± 0.226
4.527LeuSer: 4.527 ± 0.251
4.584LeuThr: 4.584 ± 0.301
4.736LeuVal: 4.736 ± 0.268
0.628LeuTrp: 0.628 ± 0.116
3.005LeuTyr: 3.005 ± 0.218
0.0LeuXaa: 0.0 ± 0.0
Met
2.244MetAla: 2.244 ± 0.189
0.323MetCys: 0.323 ± 0.074
1.293MetAsp: 1.293 ± 0.161
1.693MetGlu: 1.693 ± 0.195
1.293MetPhe: 1.293 ± 0.157
1.312MetGly: 1.312 ± 0.195
0.285MetHis: 0.285 ± 0.07
1.598MetIle: 1.598 ± 0.172
2.396MetLys: 2.396 ± 0.244
2.13MetLeu: 2.13 ± 0.203
0.894MetMet: 0.894 ± 0.156
1.636MetAsn: 1.636 ± 0.164
0.818MetPro: 0.818 ± 0.121
0.78MetGln: 0.78 ± 0.129
1.008MetArg: 1.008 ± 0.143
1.94MetSer: 1.94 ± 0.189
1.674MetThr: 1.674 ± 0.205
1.56MetVal: 1.56 ± 0.16
0.209MetTrp: 0.209 ± 0.065
0.951MetTyr: 0.951 ± 0.123
0.0MetXaa: 0.0 ± 0.0
Asn
3.823AsnAla: 3.823 ± 0.248
0.418AsnCys: 0.418 ± 0.096
3.1AsnAsp: 3.1 ± 0.3
3.918AsnGlu: 3.918 ± 0.229
2.682AsnPhe: 2.682 ± 0.238
4.127AsnGly: 4.127 ± 0.405
0.97AsnHis: 0.97 ± 0.125
3.975AsnIle: 3.975 ± 0.277
4.203AsnLys: 4.203 ± 0.293
4.051AsnLeu: 4.051 ± 0.279
1.636AsnMet: 1.636 ± 0.181
3.462AsnAsn: 3.462 ± 0.248
2.606AsnPro: 2.606 ± 0.239
1.579AsnGln: 1.579 ± 0.179
2.244AsnArg: 2.244 ± 0.169
3.386AsnSer: 3.386 ± 0.257
3.081AsnThr: 3.081 ± 0.231
3.328AsnVal: 3.328 ± 0.283
0.609AsnTrp: 0.609 ± 0.098
2.225AsnTyr: 2.225 ± 0.239
0.0AsnXaa: 0.0 ± 0.0
Pro
2.225ProAla: 2.225 ± 0.222
0.418ProCys: 0.418 ± 0.083
2.111ProAsp: 2.111 ± 0.181
3.271ProGlu: 3.271 ± 0.31
1.674ProPhe: 1.674 ± 0.171
2.282ProGly: 2.282 ± 0.213
0.628ProHis: 0.628 ± 0.135
2.149ProIle: 2.149 ± 0.177
2.511ProLys: 2.511 ± 0.22
2.282ProLeu: 2.282 ± 0.225
0.666ProMet: 0.666 ± 0.113
2.187ProAsn: 2.187 ± 0.225
1.027ProPro: 1.027 ± 0.165
1.084ProGln: 1.084 ± 0.145
1.388ProArg: 1.388 ± 0.197
2.244ProSer: 2.244 ± 0.202
2.282ProThr: 2.282 ± 0.18
2.701ProVal: 2.701 ± 0.22
0.742ProTrp: 0.742 ± 0.133
1.388ProTyr: 1.388 ± 0.169
0.0ProXaa: 0.0 ± 0.0
Gln
2.454GlnAla: 2.454 ± 0.264
0.323GlnCys: 0.323 ± 0.078
1.712GlnAsp: 1.712 ± 0.211
2.339GlnGlu: 2.339 ± 0.212
1.426GlnPhe: 1.426 ± 0.16
1.959GlnGly: 1.959 ± 0.205
0.59GlnHis: 0.59 ± 0.118
2.796GlnIle: 2.796 ± 0.231
2.492GlnLys: 2.492 ± 0.223
2.682GlnLeu: 2.682 ± 0.226
0.989GlnMet: 0.989 ± 0.149
1.465GlnAsn: 1.465 ± 0.159
1.084GlnPro: 1.084 ± 0.132
1.084GlnGln: 1.084 ± 0.167
1.693GlnArg: 1.693 ± 0.162
1.426GlnSer: 1.426 ± 0.147
1.902GlnThr: 1.902 ± 0.165
2.473GlnVal: 2.473 ± 0.249
0.742GlnTrp: 0.742 ± 0.114
1.636GlnTyr: 1.636 ± 0.177
0.0GlnXaa: 0.0 ± 0.0
Arg
2.777ArgAla: 2.777 ± 0.252
0.552ArgCys: 0.552 ± 0.117
2.701ArgAsp: 2.701 ± 0.215
3.29ArgGlu: 3.29 ± 0.288
2.111ArgPhe: 2.111 ± 0.236
2.644ArgGly: 2.644 ± 0.224
0.742ArgHis: 0.742 ± 0.12
3.252ArgIle: 3.252 ± 0.276
3.481ArgLys: 3.481 ± 0.295
3.405ArgLeu: 3.405 ± 0.234
1.198ArgMet: 1.198 ± 0.187
1.959ArgAsn: 1.959 ± 0.18
1.369ArgPro: 1.369 ± 0.169
1.75ArgGln: 1.75 ± 0.204
2.149ArgArg: 2.149 ± 0.23
2.72ArgSer: 2.72 ± 0.236
2.53ArgThr: 2.53 ± 0.222
2.796ArgVal: 2.796 ± 0.237
0.514ArgTrp: 0.514 ± 0.106
1.731ArgTyr: 1.731 ± 0.205
0.0ArgXaa: 0.0 ± 0.0
Ser
3.595SerAla: 3.595 ± 0.259
0.628SerCys: 0.628 ± 0.1
3.937SerAsp: 3.937 ± 0.295
3.975SerGlu: 3.975 ± 0.323
2.834SerPhe: 2.834 ± 0.208
4.317SerGly: 4.317 ± 0.416
1.179SerHis: 1.179 ± 0.161
4.774SerIle: 4.774 ± 0.26
4.774SerLys: 4.774 ± 0.314
4.812SerLeu: 4.812 ± 0.311
1.445SerMet: 1.445 ± 0.158
2.986SerAsn: 2.986 ± 0.244
2.358SerPro: 2.358 ± 0.246
2.168SerGln: 2.168 ± 0.18
2.91SerArg: 2.91 ± 0.303
4.736SerSer: 4.736 ± 0.367
4.26SerThr: 4.26 ± 0.307
4.222SerVal: 4.222 ± 0.297
0.875SerTrp: 0.875 ± 0.13
2.625SerTyr: 2.625 ± 0.219
0.0SerXaa: 0.0 ± 0.0
Thr
3.899ThrAla: 3.899 ± 0.341
0.59ThrCys: 0.59 ± 0.1
3.994ThrAsp: 3.994 ± 0.314
4.26ThrGlu: 4.26 ± 0.283
2.549ThrPhe: 2.549 ± 0.254
4.108ThrGly: 4.108 ± 0.344
1.274ThrHis: 1.274 ± 0.189
4.336ThrIle: 4.336 ± 0.337
4.508ThrLys: 4.508 ± 0.327
4.298ThrLeu: 4.298 ± 0.328
1.198ThrMet: 1.198 ± 0.152
2.872ThrAsn: 2.872 ± 0.221
2.986ThrPro: 2.986 ± 0.314
1.845ThrGln: 1.845 ± 0.237
2.53ThrArg: 2.53 ± 0.237
3.576ThrSer: 3.576 ± 0.351
3.633ThrThr: 3.633 ± 0.296
4.603ThrVal: 4.603 ± 0.425
0.951ThrTrp: 0.951 ± 0.137
2.701ThrTyr: 2.701 ± 0.229
0.0ThrXaa: 0.0 ± 0.0
Val
4.375ValAla: 4.375 ± 0.292
0.856ValCys: 0.856 ± 0.127
4.508ValAsp: 4.508 ± 0.309
5.383ValGlu: 5.383 ± 0.315
2.682ValPhe: 2.682 ± 0.232
3.633ValGly: 3.633 ± 0.321
1.103ValHis: 1.103 ± 0.144
4.736ValIle: 4.736 ± 0.293
5.345ValLys: 5.345 ± 0.326
4.603ValLeu: 4.603 ± 0.293
1.674ValMet: 1.674 ± 0.202
3.823ValAsn: 3.823 ± 0.251
2.054ValPro: 2.054 ± 0.171
2.435ValGln: 2.435 ± 0.222
3.138ValArg: 3.138 ± 0.253
4.546ValSer: 4.546 ± 0.308
3.956ValThr: 3.956 ± 0.287
4.793ValVal: 4.793 ± 0.288
0.818ValTrp: 0.818 ± 0.116
2.644ValTyr: 2.644 ± 0.227
0.0ValXaa: 0.0 ± 0.0
Trp
0.818TrpAla: 0.818 ± 0.11
0.133TrpCys: 0.133 ± 0.049
0.875TrpAsp: 0.875 ± 0.123
0.742TrpGlu: 0.742 ± 0.118
0.666TrpPhe: 0.666 ± 0.11
0.456TrpGly: 0.456 ± 0.111
0.114TrpHis: 0.114 ± 0.058
1.065TrpIle: 1.065 ± 0.159
1.388TrpLys: 1.388 ± 0.149
0.875TrpLeu: 0.875 ± 0.128
0.437TrpMet: 0.437 ± 0.089
0.951TrpAsn: 0.951 ± 0.123
0.495TrpPro: 0.495 ± 0.111
0.533TrpGln: 0.533 ± 0.092
0.418TrpArg: 0.418 ± 0.092
0.837TrpSer: 0.837 ± 0.124
0.913TrpThr: 0.913 ± 0.124
0.932TrpVal: 0.932 ± 0.112
0.19TrpTrp: 0.19 ± 0.058
0.723TrpTyr: 0.723 ± 0.129
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.024TyrAla: 3.024 ± 0.229
0.552TyrCys: 0.552 ± 0.115
2.948TyrAsp: 2.948 ± 0.195
2.834TyrGlu: 2.834 ± 0.253
1.921TyrPhe: 1.921 ± 0.191
2.511TyrGly: 2.511 ± 0.214
0.799TyrHis: 0.799 ± 0.121
3.024TyrIle: 3.024 ± 0.233
3.024TyrLys: 3.024 ± 0.25
2.91TyrLeu: 2.91 ± 0.228
1.179TyrMet: 1.179 ± 0.151
2.568TyrAsn: 2.568 ± 0.23
1.712TyrPro: 1.712 ± 0.186
1.541TyrGln: 1.541 ± 0.152
1.579TyrArg: 1.579 ± 0.155
2.644TyrSer: 2.644 ± 0.221
2.625TyrThr: 2.625 ± 0.228
3.195TyrVal: 3.195 ± 0.249
0.609TyrTrp: 0.609 ± 0.096
1.75TyrTyr: 1.75 ± 0.211
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 266 proteins (52578 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski